BS EN 1390-2020 pdf free download.Wood preservatives — Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae — Laboratory method
4 Principle
Insertion of larvae of Hylotrupes bajulus into test specimens. After a period of time to allow the larvae to establish themselves in the test specimens, treatment of these test specimens by brushing or pipetting of the test preservative product.
After the time defined by the speed of action of the wood preservative, assessment of the mortality of the larvae compared with that of larvae in untreated control test specimens.
5 Test materials
5.1 Biological material
5.1.1 Hylotrupes bajulus (Linnaeus) larvae
5.1.2 Source of larvae
The larvae shall preferably be obtained from cultures reared according to the method described in Annex B.
Larvae can also be taken from naturally infested wood, in which case they should be transferred into sapwood of pine and stored for at least four weeks under the rearing conditions specified in Annex B.
5.1.3 Provision of larvae
Carefully split or crumble infested blocks to extract larvae.
Keep the larvae separate from one another in glass receptacles for two or three days in the culturing chamber (5.3.1) to check they are healthy.
5.1.4 Choice of larvae
Use only healthy larvae in the test.
NOTE A healthy larva can be recognized by ivory-white colour, its firm consistency and rounded appearance, and by the absence of wounds or bites, which show up as dark marks. Healthy larvae react to the touch by vigorous movement and attempts to bite.
Reject any larvae, which are shrunken or aged which have recently moulted, or which are in a prepupal stage.
Weigh each larva and place it in a glass receptacle marking the receptacle with the weight of the larva. Make up two groups with the weight ranges:
— 51 mg to 100 mg, and
— 101 mg to 150 mg.
Larvae with a mass larger than 150 mg in mass are unsuitable as they can pupate during the course of the test.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which water is the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found suitable.
5.2.2 Gelatine, for sealing the relevant surfaces of specimens to be treated with solutions in which an organic solvent is the continuous phase.
5.2.3 Water, complying with grade 3 of EN ISO 3696.
5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (28 ± 2) °C and at a relative humidity of (70 ± 5) %.
5.3.2 Ventilated fume cupboard, in which the specimens are treated with an input air temperature of (20 ± 5) °C.
The maximum air speed, measured at the input opening with the sash in the approximate operation position should be approx. 0,5 m/s.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their vapours.
5.3.3 Testing chamber, ventilated and controlled at (21 ± 2) °C and at a relative humidity of (75 ± 5) %.
5.3.4 Drill and twist drills, with 3 mm, 4 mm and 5 mm diameters.
5.3.5 Pipettes as specified in ISO 835:2007, Class B – graduated pipette with no waiting time,with a capacity 5 ml and an accuracy of ±0,05 ml.
5.3.6 Brush of appropriate size, to be used when the test substance is applied by brushing (8.5.2).
5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test,to ensure the safety of the operator (see also Annex C).
5.3.8 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of ±1 mg.
5.3.9 Rectangular cover with sides, constructed either of glass, plastics, plywood and of a height not less than 200 mm and with an open face of sufficient size to cover all the treated specimens from a single test.BS EN 1390 pdf download.